Endogenous RGS proteins enhance acute desensitization of GABAB receptor-activated GIRK currents in HEK-293T cells
نویسندگان
چکیده
منابع مشابه
Addictive drugs modulate GIRK-channel signaling by regulating RGS proteins.
Regulator of G-protein signaling (RGS) proteins are strong modulators of G-protein-mediated pathways in the nervous system. One function of RGS proteins is to accelerate the activation-deactivation kinetics of G-protein-coupled inwardly rectifying potassium (GIRK) channels. The opening of GIRK channels reduces the firing rates of neurons. Recent studies indicate that RGS proteins also modulate ...
متن کاملGIRK channel modulation by assembly with allosterically regulated RGS proteins.
G-protein-activated inward-rectifying K(+) (GIRK) channels hyperpolarize neurons to inhibit synaptic transmission throughout the nervous system. By accelerating G-protein deactivation kinetics, the regulator of G-protein signaling (RGS) protein family modulates the timing of GIRK activity. Despite many investigations, whether RGS proteins modulate GIRK activity in neurons by mechanisms involvin...
متن کاملEvidence that the nucleotide exchange and hydrolysis cycle of G proteins causes acute desensitization of G-protein gated inward rectifier K+ channels.
The G-protein gated inward rectifier K+ channel (GIRK) is activated in vivo by the Gbeta gamma subunits liberated upon Gi-coupled receptor activation. We have recapitulated the acute desensitization of receptor-activated GIRK currents in heterologous systems and shown that it is a membrane-delimited process. Its kinetics depends on the guanine nucleotide species available and could be accounted...
متن کاملShort-term desensitization of G-protein-activated, inwardly rectifying K+ (GIRK) currents in pyramidal neurons of rat neocortex.
Whole cell recordings from acutely isolated rat neocortical pyramidal cells were performed to study the kinetics and the mechanisms of short-term desensitization of G-protein-activated, inwardly rectifying K+ (GIRK) currents during prolonged application (5 min) of baclofen, adenosine, or serotonin. Most commonly, desensitization of GIRK currents was characterized by a biphasic time course with ...
متن کاملG protein-activated inwardly rectifying K+ (GIRK) currents in dendrites of rat neocortical pyramidal cells.
1. We performed patch-clamp recordings on acutely isolated dendritic segments and cell somata of rat neocortical pyramidal neurons to determine and compare the relative density of G protein-activated K+ (GIRK) currents in the two cellular compartments. 2. Hyperpolarizing voltage ramps and elevation of extracellular K+ concentration to 40 mM served to identify inwardly rectifying K+ currents. Ne...
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ژورنال
عنوان ژورنال: Pfl�gers Archiv - European Journal of Physiology
سال: 2004
ISSN: 0031-6768,1432-2013
DOI: 10.1007/s00424-004-1367-1